Anti-FUT8 antibody (bs-6280R)

Anti-FUT8 antibody

DATASHEET

Host:Rabbit

Target Protein:FUT8

IR:Immunogen Range:311-410/575

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:2530

Swiss Prot:Q9BYC5

Source:KLH conjugated synthetic peptide derived from human FUT8:311-410/575 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans.The expression of this gene may contribute to the malignancy of cancer cells and to their invasive and metastatic capabilities. There are 2 isoforms produced by alternative splicing. Immunogen is not present in isoform 2 (also known as Retinal).

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Pig
Cow
Horse
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FUT8) Polyclonal Antibody, Unconjugated (bs-6280R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.