Anti-RRBP1 antibody
| 英文名称 | RRBP1 |
| 中文名称 | 核糖体结合蛋白1抗体 |
| 别 名 | 180 kDa ribosome receptor homolog; DKFZp586A1420; ES/130; ES/130 related protein; ES130; FLJ36146; hES; Ribosome binding protein 1; Ribosome binding protein 1 homolog 180kDa; Ribosome binding protein 1 homolog; Ribosome receptor protein; RRBP 1; RRp; RRBP1_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:RRBP1
IR:Immunogen Range:641-740/1410
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:6238
Swiss Prot:Q9P2E9
Source:KLH conjugated synthetic peptide derived from human RRBP1:641-740/1410
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:RRBP1 (Ribosome-binding protein 1) acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
Flow-Cyt(3ug/Test)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-RRBP1 antibody (bs-6574R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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