Anti-Laminin 5 antibody
| 英文名称 | Laminin 5 |
| 中文名称 | 层粘连蛋白5抗体 |
| 别 名 | E170; Epiligrin 170 kDa subunit; Epiligrin subunit alpha; Kalinin subunit alpha; LAMA3; LAMA3A; Laminin 5 alpha3; Laminin A3; laminin alpha 3; laminin alpha3; Laminin subunit alpha-3; Laminin-5 subunit alpha; Laminin-6 subunit alpha; Laminin-7 subunit alpha; laminin5 alpha 3; Laminin5 alpha3; LAMNA; LOCS; Nicein subunit alpha; LAMA3_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:Laminin 5
IR:Immunogen Range:2701-2900/3333
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:3909
Swiss Prot:Q16787
Source:KLH conjugated synthetic peptide derived from human Laminin 5:2701-2900/3333
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminin-5 is thought to be involved in (1) cell adhesion via integrin alpha-3/beta-1 in focal adhesion and integrin alpha-6/beta-4 in hemidesmosomes, (2) signal transduction via tyrosine phosphorylation of pp125-FAK and p80, (3) differentiation of keratinocytes.
Tissue specificity; Skin; respiratory, urinary, and digestive epithelia and in other specialized tissues with prominent secretory or protective functions. Epithelial basement membrane, and epithelial cell tongue that migrates into a wound bed. A differential and focal expression of the subunit alpha-3 is observed in the CNS.
Size:100ul
Concentration:1mg/ml
Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Tissue/cell: mouse stomach wall; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Laminin 5 Polyclonal Antibody, Unconjugated(bs-6713R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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