Anti-phospho-TIRAP (Tyr86) antibody
| 英文名称 | phospho-TIRAP (Tyr86) |
| 中文名称 | 磷酸化白细胞介素1受体衔接蛋白抗体 |
| 别 名 | TIRAP (phospho Y86); Adapter protein wyatt; MAL antibody MyD88 adapter like protein; TIR domain containing adapter protein; Toll interleukin 1 receptor (TIR) domain containing adaptor protein; Toll interleukin 1 receptor domain containing adapter protein; Toll like receptor adaptor protein; Wyatt. |
DATASHEET
Host:Rabbit
Target Protein:phospho-TIRAP (Tyr86)
IR:Immunogen Range:KD(p-Y)DV
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:114609
Swiss Prot:P58753
Source:KLH conjugated Synthesised phosphopeptide derived from human TIRAP around the acetylation site of Tyr86:KD(p-Y)DV
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The Toll-like receptor (TLR) family in mammals comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. TIRAP (TIR domain-containing adaptor protein) is an adaptor protein used by TLR4. Blocking TIRAP inhibits TLR4-mediated signaling events, including DC maturation and cytokine production.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(2ug/Test)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Dog
Pig
Cow
Horse
Guinea Pig
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Blank control:U937.
Primary Antibody (green line): Rabbit Anti-phospho-TIRAP(Tyr86) antibody (bs-7562R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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