Anti-RHBDD3 antibody (bs-7566R)

Anti-RHBDD3 antibody

DATASHEET

Host:Rabbit

Target Protein:RHBDD3

IR:Immunogen Range:151-250/386

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:25807

Swiss Prot:Q9Y3P4

Source:KLH conjugated synthetic peptide derived from human RHBDD3:151-250/386 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:PTAG is a novel 386 amino acid multi-pass membrane protein that contains one UBA domain and augments drug-induced apoptosis. Cells lacking PTAG have a reduced apoptotic response, thereby causing a predisposition to cell malignancy and resistance to chemotherapeutic interventions, and PTAG plays a role in colorectal tumorigenesis as the majority of primary colorectal tumors lack the PTAG gene. Encoded by a gene located on human chromosome 22, PTAG is often co-expressed with EWS (ewing sarcoma breakpoint region 1), a gene located directly downstream of PTAG.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Testis (Mouse) Lysate at 40 ug
Heart (Mouse) Lysate at 40 ug
Liver (Mouse) Lysate at 40 ug
Placenta (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
Cerebrum (Mouse) Lysate at 40 ug
Skeletal muscle (Mouse) Lysate at 40 ug
A431(Human) Cell Lysate at 30 ug
HL60(Human) Cell Lysate at 30 ug
A549(Human) Cell Lysate at 30 ug
Molt-4(Human) Cell Lysate at 30 ug
Primary: Anti- RHBDD3 (bs-7566R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33’51 kD
Observed band size: 51 kD