Anti-Corin antibody (bs-7685R)

Anti-Corin antibody 

DATASHEET

Host:Rabbit

Target Protein:Corin

IR:Immunogen Range:165-245/1042

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:10699

Swiss Prot:Q9Y5Q5

Source:KLH conjugated synthetic peptide derived from human Corin:165-245/1042 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Corin was first described as a trypsin-like serine proteinase in the heart which processed pro-atrial natriuretic peptide into the active form ANP. Corin cleaves pro-ANP at Arg98/Ser-99, and blocking Corin message inhibits pro-ANP processing. Null mutant mice lacking Corin develop hypertension, and a series of missense mutations have been identified in humans with high blood pressure and hypertension. Corin is a member of the type-II transmembrane serine proteinases, such as Hepsin, Epitheliasin, Matriptase and enteropeptidase. The Corin domain structure includes a cytoplasmic domain, a transmembrane domain, a spacer region, a frizzled-like domain, 5 LDL Receptor-like domains, another frizzled-like domain, 3 more LDLR-like repeats, a SRCR (Scavenger Receptor-Cysteine Rich) domain (also called macrophage scavenger-like domain), and finally the trypsin-like serine proteinase domain. The complex domain structure puts Corin into the “mosaic” TMPRSS family, so named because of the wide variety of domains.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Horse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Tissue/cell: rat cardiac muscle; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Corin Polyclonal Antibody, Unconjugated(bs-7685R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining