Anti-NEK1 antibody (bs-7814R)

Anti-NEK1 antibody

DATASHEET

Host:Rabbit

Target Protein:NEK1

IR:Immunogen Range:301-400/1258

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:4750

Swiss Prot:Q96PY6

Source:KLH conjugated synthetic peptide derived from human NEK1:301-400/1258 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The protein encoded by this gene is a serine/threoninekinase involved in cell cycle regulation. The encoded protein isfound in a centrosomal complex with FEZ1, a neuronal protein thatplays a role in axonal development. Defects in this gene are acause of polycystic kidney disease (PKD). Several transcriptvariants encoding different isoforms have been found for this gene.[provided by RefSeq, Dec 2010].

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Cow
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (NEK1) Polyclonal Antibody, Unconjugated (bs-7814R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.