Anti-RABGAP1 antibody (bs-7826R)

Anti-RABGAP1 antibody 

DATASHEET

Host:Rabbit

Target Protein:RABGAP1

IR:Immunogen Range:901-1000/1069

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:23637

Swiss Prot:Q9Y3P9

Source:KLH conjugated synthetic peptide derived from human RABGAP1:901-1000/1069 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Rab GTPase-activating protein 1 is a 1,069 amino acid cytoplasmic protein containing one PID domain and one Rab-GAP TBC domain. Rab GAP1 acts as a GTPase-activating protein of Rab 6A in the Rab 6A-mediated pathway, which is involved in the metaphase-anaphase transition. Expressed as four alternatively spliced variants, Rab GAP1 may also be involved in the microtubule nucleation by centrosome and in Golgi dynamics during the cell cycle. Rab GAP1 is encoded by a gene located on human chromosome 9, which consists of about 145 million bases, 4% of the human genome and encodes nearly 900 genes.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RABGAP1) Polyclonal Antibody, Unconjugated (bs-7826R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.