Anti-CCDC34 antibody (bs-8098R)

Anti-CCDC34 antibody 

DATASHEET

Host:Rabbit

Target Protein:CCDC34

IR:Immunogen Range:177-280/373

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:91057

Swiss Prot:Q96HJ3

Source:KLH conjugated synthetic peptide derived from human CCDC34:177-280/373 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The coiled-coil domain is a structural motif found in proteins that are involved in a diverse array of biological functions such as the regulation of gene expression, cell division, membrane fusion and drug extrusion and delivery. CCDC34 (coiled-coil domain containing 34), also known as Renal carcinoma antigen NY-REN-41, is a 373 amino acid protein that is expressed in testis, breast, lung, placenta, liver and small intestine. A translocation between the short arms of chromosomes 11 and 18 affecting the CCDC34 gene has been identified in a patient with hamartoma of the retinal pigment epithelium. There are two isoforms of CCDC34 that are produced as a result of alternative splicing events.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Pig
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CCDC34 Polyclonal Antibody, Unconjugated(bs-8098R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining