Anti-DCAKD antibody (bs-8249R)

Anti-DCAKD antibody 

DATASHEET

Host:Rabbit

Target Protein:DCAKD

IR:Immunogen Range:61-160/231

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:79877

Swiss Prot:Q8WVC6

Source:KLH conjugated synthetic peptide derived from human DCAKD:61-160/231 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:DCAKD belongs to the coaE family. It contains one DPCK (dephospho CoA kinase) domain. There are two isoforms.Coenzyme A (CoA) is an essential cofactor used in numerous biochemical pathways. It plays a critical role in the synthesis and oxidation of fatty acids and is vital to the citric acid cycle. The biosynthesis pathway of CoA from pantothenic acid (also known as vitamin B5) is essential and universal in prokaryotes and eukaryotes. In humans, the final steps of the biosynthesis pathway are carried out by the bifunctional enzyme COASY. The sequence of these enzymes are highly conserved between different bacterial species. The phosphopantetheine adenylyltransferase and dephospho-coenzyme A kinase activities of COASY are evolutionarily conserved activities. DCAKD (dephospho-CoA kinase domain containing protein) is a 231 amino acid protein that consists of a dephospho-CoA kinase domain and an ATP nucleotide binding motif. Localizing to mitochondria and the cytosol, DCAKD belongs to the coaE family which suggests that it may play a role in the biosynthesis of CoA.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Molt-4(Human) Cell Lysate at 30 ug
Primary: Anti- DCAKD (bs-8249R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 27 kD