Anti-RFPL2+RFPL3 antibody (bs-8493R)

Anti-RFPL2+RFPL3 antibody

DATASHEET

Host:Rabbit

Target Protein:RFPL2+RFPL3

IR:Immunogen Range:141-240/378

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:10739

Swiss Prot:O75678

Source:KLH conjugated synthetic peptide derived from human RFPL3:141-240/378 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The RFPL3 protein is 371 amino acids long and may have been emerged due to intrachromosomal duplication.RFPL1, RFPL2 and RFPL3 (ret finger protein-like 1, 2 and 3, respectively), exist as a cluster of genes mapping to human chromosome 22q12.2-q13.3, sharing 95%-96% identity. RFPL1, 2 and 3, are thought to contribute to neocortex organization and size in primates, and show high expression in fetal neocortex as well as embryonic stem-cell neurogenesis. Each of the three RFPL genes encodes two exons giving rise to a putative RING-like motifs and B30-2 domains. RFPL1, also known as RNF78 or MGC132428, is a 317 amino acid protein known to have high expression in prostate with lower expression in adult brain, fetal liver and fetal kidney. RFPL2, or RNF79, is 378 amino acids long and is also highly expressed in prostate with lower expression in fetal kidney and fetal liver. As a result of alternative splicing, three isoforms of RFPL2 exist.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)

Cross Reactive Species:Human
Mouse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti- RFPL2+RFPL3 (bs-8493R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 45 kD