Anti-RNF40 antibody
| 英文名称 | RNF40 |
| 中文名称 | 环指蛋白40抗体 |
| 别 名 | BRE1 E3 ubiquitin ligase homolog B; BRE1-B; BRE1B; BRE1B_HUMAN; E3 ubiquitin protein ligase BRE1B; E3 ubiquitin-protein ligase BRE1B; Rb associated protein; RBP 95; RBP95; Ring finger protein 40; RNF 40; Rnf40; STARING; 95 kDa retinoblastoma associated protein; 95 kDa retinoblastoma protein binding protein; 95 kDa retinoblastoma-associated protein; BRE 1B; BRE1 B. |
DATASHEET
Host:Rabbit
Target Protein:RNF40
IR:Immunogen Range:901-1001/1001
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:9810
Swiss Prot:O75150
Source:KLH conjugated synthetic peptide derived from human RNF40:901-1001/1001
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). RNF40 (RING finger protein 40), also known as BRE1B, Staring or RBP95, is a 1,001 amino acid nuclear protein that contains one RING-type zinc finger. Expressed ubiquitously with highest expression in heart, testis and pancreas, RNF40 functions as an E3 ubiquitin-protein ligase that regulates the monoubiquitination and subsequent degradation of select residues on target proteins, such as Histone H2B and Syntaxin 1. In addition, RNF40 forms a ubiquitin ligase complex with UBCH6 (an E2 enzyme) and together, these proteins play a crucial role in regulation of the histone code. Four isoforms of RNF40 exist due to alternative splicing events.
Size:100ul
Concentration:1mg/ml
Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:50-200)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-RNF40 Polyclonal Antibody, Unconjugated(bs-9170R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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