Anti-BEND5 antibody (bs-9270R)

Anti-BEND5 antibody 

DATASHEET

Host:Rabbit

Target Protein:BEND5

IR:Immunogen Range:31-130/421

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:79656

Swiss Prot:Q7L4P6

Source:KLH conjugated synthetic peptide derived from human BEND5:31-130/421 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(2ug/Test)
IF(1:50-200)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Cow
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Blank control:Mouse spleen.
Primary Antibody (green line): Rabbit Anti-BEND5 antibody (bs-9270R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ethanol (10min at room temperature) and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.