Anti-LRCH4/LRRN1 antibody (bs-9330R)

Anti-LRCH4/LRRN1 antibody

DATASHEET

Host:Rabbit

Target Protein:LRCH4/LRRN1

IR:Immunogen Range:31-130/683

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:4034

Swiss Prot:O75427

Source:KLH conjugated synthetic peptide derived from human LRCH4:31-130/683 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Members of the leucine-rich repeat family include LRCH1, LRCH2, LRCH3 and LRCH4. All family members contain one calponin-homology domain and nine leucine-rich repeats. LRCH4 (leucine-rich repeats and calponin homology (CH) domain containing 4), also known as LRN, LRRN1, LRRN4 or SAP25, is a 683 amino acid protein that belongs to the leucine-rich repeat family. The carboJNC-terminus of LRCH4 may act as a membrane anchor between cells, while the amino-terminus contains the leucine-rich domains, which is thought to be involved in ligand binding. The calponin homology (CH) domain is suggested to confer Actin binding to a variety of cytoskeletal and signaling molecules. The gene encoding LRCH4 maps to human chromosome 7, which houses over 1,000 genes and comprises nearly 5% of the human genome.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:50-200)

Cross Reactive Species:Human
Mouse
Rat
Pig
Cow
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Tissue/cell: Rat stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-LRCH4 Polyclonal Antibody, Unconjugated(bs-9330R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining