Anti-Proteasome 20S alpha 6 antibody (bs-9355R)

Anti-Proteasome 20S alpha 6 antibody 

DATASHEET

Host:Rabbit

Target Protein:Proteasome 20S alpha 6

IR:Immunogen Range:1-100/246

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:5687

Swiss Prot:P60900

Source:KLH conjugated synthetic peptide derived from human Proteasome 20S alpha 6:1-100/246 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The gene encodes a 246-amino acid polypeptide containing an RNA binding motif, a putative nuclear localization signal, and phosphorylation sites. The alpha subunits comprises the outer rings of the proteasome. Some alpha subunits contain a functional nuclear localization signal; proteasomes are found in both the nuclear and cytoplasmic compartments of the cell. Alpha subunits may constitute a physical barrier that limits access of cytosolic proteins into the inner proteolytic chamber.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:50-200)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Horse
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PSMA6) Polyclonal Antibody, Unconjugated (bs-9355R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.