Anti-NOP2 antibody
英文名称 | NOP2 |
中文名称 | 增殖相关核仁抗原抗体 |
别 名 | Proliferation Associated Nucleolar Antigen: NOL 1; NUCLEOLAR ANTIGEN p120; NOP2_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:NOP2
IR:Immunogen Range:1-100/812
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:4839
Swiss Prot:P46087
Source:KLH conjugated synthetic peptide derived from human NOL1:1-100/812
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Proliferation-associated nucleolar antigen is expressed in mid G1 phase with peak level during the S phase and a rapid degradation during late mitosis. Its expression in breast carcinoma is correlated with patient prognosis.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:50-200)
Cross Reactive Species:Human
Mouse
Rat
Dog
Cow
Horse
Rabbit
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NOP2) Polyclonal Antibody, Unconjugated (bs-9710R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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