Anti-CHPT1 antibody (bs-9725R)

Anti-CHPT1 antibody 

DATASHEET

Host:Rabbit

Target Protein:CHPT1

IR:Immunogen Range:61-160/406

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:56994

Swiss Prot:Q8WUD6

Source:KLH conjugated synthetic peptide derived from human CHPT1:61-160/406 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:CHPT1, also known as AAPT1-like protein and Diacylglycerol cholinephosphotransferase 1, is a 406 amino acid multi-pass membrane protein that is localized to the golgi apparatus. By catalyzing the phosphatidylcholine biosynthesis from CDP-choline, it plays an essential role in the formation and maintenance of vesicular membranes. CHPT1 is most abundant in testis, as well as small intestine, heart, colon, spleen and prostate. Expression of CHPT1 is increased in cancerous breast cells as compared to normal breast cell lines and it has been determined that the CHPT1 gene exhibits mutations within the cancerous cells. Interestingly, exposure to mustard gas significantly decreases CHPT1 gene expression and activity, an event that may play an important role in the development of acute respiratory distress syndrome (ARDS). There are two isoforms of CHPT1 that are produced as a result of alternative splicing events.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Cerebrum (Mouse) Lysate at 40 ug
Heart (Mouse) Lysate at 40 ug
Primary: Anti-CHPT1 (bs-9725R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28'50 kD
Observed band size: 50 kD