Anti-SAP62 antibody (bs-6997R)

Anti-SAP62 antibody

DATASHEET

Host:Rabbit

Target Protein:SAP62

IR:Immunogen Range:65-160/464

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:8175

Swiss Prot:Q15428

Source:KLH conjugated synthetic peptide derived from human SAP62:65-160/464 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:SAP 62, also known as SF3A2 (Splicing factor 3A subunit 2), PRP11, PRPF11 or SF3a66, is a 464 amino acid protein that contains one matrin-type zinc finger and belongs to the SF3A2 family. Localized to the nucleus, SAP 62 is a subunit of the SF3A splicing factor, a heterotrimeric complex comprised of three subunits that act in tandem to mediate the binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. The SF3A complex is necessary for the conversion of 15S U2 snRNP into the active 17S protein that performs directly in pre-mRNA splicing events. Functioning as the second subunit of the complex, SAP 62 interacts with subunit 1 (SAP 114) via its N-terminus while simultaneously binding to 15S U2 snRNP via its zinc finger domain. In addition to its role in RNA splicing, SAP 62 is thought to act independently as a microtubule-binding protein.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:50-200)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Cow
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SAP62 Polyclonal Antibody, Unconjugated(bs-6997R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining