Anti-5 MethylCytosine antibody (bs-9450R)

Anti-5 MethylCytosine antibody

DATASHEET

Host:Rabbit

Target Protein:5 MethylCytosine

IR:Immunogen Range:

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:58366-64-6

Swiss Prot:N/A

Source:KLH conjugated 5 MethylCytosine: 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Cytidine is a nucleoside formed by a cytosine attached to a ribose ring via a beta-N1-glycosidic bond. DNA is methylated on cytidines by DNA methylases (DNMTs)to generate 5-methylcytidine (5-mC), a potent epigenetics marker and regulator of gene expression. The reverse reaction (cytidine demethylation) starts with its oxidation to hydroJNCmethyl- (5-hmC), formyl- (5-fC), and carboJNC- (5-caC) cytidine. Several enzymes, including the Tet family of proteins have been implicated in cytidine demethylation.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
Flow-Cyt(1ug/Test)

Cross Reactive Species:Human
5 MethylCytosine.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Blank control:Hela.
Primary Antibody (green line): Rabbit Anti-5 MethylCytosine antibody (bs-9450R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.