Anti-ECRG4 antibody (bs-9807R)

Anti-ECRG4 antibody

DATASHEET

Host:Rabbit

Target Protein:ECRG4

IR:Immunogen Range:41-148/148

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:84417

Swiss Prot:Q9H1Z8

Source:KLH conjugated synthetic peptide derived from human ECRG4/C2orf40:41-148/148 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:ECRG4, also known as augurin or C2orf40, is a 148 amino acid secreted protein. Belonging to the augurin family, ECRG4 is thought to be a hormone. It has also been suggested that ECRG4 may act as a tumor suppressor. The gene that encodes ECRG4 maps to human chromosome 2, which consists of 237 million bases encoding over 1,400 genes, making up approximately 8% of the human genome. A number of genetic diseases are linked to genes on chromosome 2. Harlequin icthyosis, a rare and morbid skin deformity, is associated with mutations in the ABCA12 gene. The lipid metabolic disorder sitosterolemia is associated with ABCG5 and ABCG8. An extremely rare recessive genetic disorder, Alstr syndrome is due to mutations in the ALMS1 gene. Interestingly, chromosome 2 contains what appears to be a vestigial second centromere and vestigial telomeres which gives credence to the hypothesis that human chromosome 2 is the result of an ancient fusion of two ancestral chromosomes.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:50-200)
IEM(1:20-200)
IGS(1:20-200)
GICA(1:20-200)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (Rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ECRG4) Polyclonal Antibody, Unconjugated (bs-9807R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.