Anti-THOC2 antibody (bs-1663R)

Anti-THOC2 antibody

DATASHEET

Host:Rabbit

Target Protein:THOC2

IR:Immunogen Range:101-200/1593

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:57187

Swiss Prot:Q8NI27

Source:KLH conjugated synthetic peptide derived from human THOC2:101-200/1593 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:THOC2 is a 1,478 amino acid nuclear protein that functions as a component of the heteromultimeric THO/TREX (transcription/export) complex, a multi-protein structure consisting of THOC1, THOC2, THOC3, BAT1 and ALY. The THO/TREX complex is recruited to transcribed genes and travels with RNA polymerase II (Pol II) during elongation, coupling elongating Pol II with RNA splicing and export factors. Defects in the protein components of the TREX structure can cause transcription impairment, mRNA export defects and transcription-associated recombination. THOC2, an important component of the TREX complex, exists as two isoforms that are produced by alternative splicing events.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:50-200)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-THOC2 Polyclonal Antibody, Unconjugated(bs-1663R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining