Anti-phospho-CstF64 (Ser83) antibody
| 英文名称 | phospho-CstF64 (Ser83) |
| 中文名称 | 磷酸化细胞分裂相关蛋白CSTF64抗体 |
| 别 名 | p-CstF-64 (Ser83); p-CstF-64 (S83); CstF64 (phospho-Ser83); CstF64 (phospho-S83); CSTF2T; CF1 64 kDa subunit, tau variant; Cleavage stimulation factor 64 kDa subunit, tau variant; CSTF 64 kDa subunit, tau variant; KIAA0689; TauCstF64; CSTF2_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:phospho-CstF64 (Ser83)
IR:Immunogen Range:EF(p-S)GR
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:1478
Swiss Prot:P33240
Source:KLH conjugated synthesised phosphopeptide derived from human CstF64 around the phosphorylation site of Ser83:EF(p-S)GR
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Polyadenylation of mRNA precursors is a two-step reaction that requires multiple protein factors. The first step, endonucleolytic cleavage of polyadenylation substrates, requires CstF (cleavage stimulation factor), a heterotrimer that is composed of three distinct subunits. CstF-64 contains an RNA binding domain and is responsible for the RNA binding activity of CstF. CstF-64 is expressed in all somatic cells and in pre- and postmeiotic, but not meiotic, germ cells. However, a large variant of CstF-64, called t CstF-64, is abundantly expressed in meiotic and postmeiotic cells in the testis and to a lesser extent in the brain, and promotes the germ cell pattern of polyadenylation. The gene encoding CstF-64 (designated CSTF2) maps to the X chromosome, whereas t CstF-64 is encoded by an autosomal gene. The increase in CstF-64 concentration during B cell activation switches IgM heavy chain mRNA expression from membrane-bound to secreted forms, suggesting that CstF-64 plays a key role in regulating IgM heavy chain expression during B cell differentiation.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample: MCF-7 Cell (Human) Lysate at 40 ug
Primary: Anti-phospho-CstF64 (Ser83) (bs-8522R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61 kD
Observed band size: 61 kD
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