Anti-HEBP1 antibody (bs-9887R)

Anti-HEBP1 antibody

DATASHEET

Host:Rabbit

Target Protein:HEBP1

IR:Immunogen Range:51-150/189

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:50865

Swiss Prot:Q9NRV9

Source:KLH conjugated synthetic peptide derived from human HEBP1/p22HBP:51-150/189 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:p22HBP, also known as HEBP1 (heme binding protein 1), HBP or HEBP, is a 189 amino acid intracellular tetrapyrrole-binding protein that assists in prevention of cellular toxicity by removing free porphyrinogens from the cell. Existing as a monomer, p22HBP localizes to cytoplasm and contains a 21 amino acid chemoattractant within its N-terminus that functions as a natural ligand for FPR3. p22HBP is a member of the HEBP family and binds N-methylprotoporphyrin and metalloporphyrins with similar affinity to porphyrinogens. The gene encoding p22HBP maps to human chromosome 12, which encodes over 1,100 genes and comprises approximately 4.5% of the human genome. Chromosome 12 is associated with a variety of diseases and afflictions, including hypochondrogenesis, achondrogenesis, Kniest dysplasia, Noonan syndrome and trisomy 12p, which causes facial developmental defects and seizure disorders.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:50-200)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Horse
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HEBP1) Polyclonal Antibody, Unconjugated (bs-9887R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.