Anti-KCNE1L antibody
| 英文名称 | KCNE1L |
| 中文名称 | 钾离子通道蛋白家族成员1样蛋白抗体 |
| 别 名 | AMME syndrome candidate gene 2 protein; AMMECR2 protein; Cardiac voltage gated potassium channel accessory subunit 5; KCNE1 like; KCNE5; Mink; MinK like protein; Potassium voltage gated channel subfamily E member 1 like protein; Potassium voltage gated channel, Isk related family, member 1 like; Voltage gated potassium channel accessory subunit 5; KCE1L_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:KCNE1L
IR:Immunogen Range:11-110/142
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:23630
Swiss Prot:Q9UJ90
Source:KLH conjugated synthetic peptide derived from human KCNE1L:11-110/142
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:KCNE1L belongs to the potassium channel KCNE family which represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1ug/test)
IF(1:50-200)
Cross Reactive Species:Human
Mouse
Rat
Pig
Cow
Rabbit
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-KCNE1L antibody (bs-9929R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min atroom temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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