Anti-Endomucin antibody (bs-4808R)

Anti-Endomucin antibody

DATASHEET

Host:Rabbit

Target Protein:Endomucin

IR:Immunogen Range:21-120/261

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:51705

Swiss Prot:Q9ULC0

Source:KLH conjugated synthetic peptide derived from human Endomucin:21-120/261 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Endothelial sialomucin, also called endomucin or mucin-like sialoglycoprotein, which interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:50-200)

Cross Reactive Species:Human
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Endomucin) Polyclonal Antibody, Unconjugated (bs-4808R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.