Anti-IL-18 antibody (bs-4986R)

Anti-IL-18 antibody

DATASHEET

Host:Rabbit

Target Protein:IL-18

IR:Immunogen Range:

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:3606

Swiss Prot:Q14116

Source:Recombinant human IL-18: 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:IL18 (IFN-gamma-inducing factor, IGIF) is a novel pro-inflammatory cytokine that augments natural killer (NK) activity in spleen cells. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL18 also has other biological properties such as stimulation of Fas ligand expression, and induction of CC and CXC chemokines. IL18 increases production of competent HIV. IL18 is related to the IL1 family. One of the IL18 receptor chains is the IL1 receptor-related protein, a member of the IL 1R family. Like IL1 , IL18 is first synthesized as a leaderless precursor requiring the IL1 converting enzyme (ICE) for cleavage into an active molecule. IL18 may play a role in tissue injury in inflammatory reactions.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)

Cross Reactive Species:Human
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lane 1: Du145 (Human) Cell Lysate at 30 ug
Lane 2: Hela (Human) Cell Lysate at 30 ug
Lane 3: U2os (Human) Cell Lysate at 30 ug
Lane 4: A431 (Human) Cell Lysate at 30 ug
Lane 5: Siha (Human) Cell Lysate at 30 ug
Primary: Anti-IL-18 (bs-4986R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 22 kD