Anti-Phospho-CHEK1 (Ser296) antibody
| 英文名称 | Phospho-CHEK1 (Ser296) |
| 中文名称 | 磷酸化细胞周期检测点激酶1抗体 |
| 别 名 | Chk1 (Phospho-Ser296); Chk1 (phospho S296);Phospho-Chk1 (Ser296); p-Chk1 (phospho S296); Phospho-Chk1(S296); Cell cycle checkpoint kinase; Checkpoint, S. pombe, homolog of, 1; Checkpoint kinase 1; Checkpoint kinase 1 homolog (S. pombe); CHEK 1; CHEK-1; CHEK1; Chk 1; Chk1; CHK1 checkpoint homolog (S. pombe); EC 2.7.11.1; rad27; Serine/threonine protein kinase Chk1; Serine/threonine-protein kinase CHK1; STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae); C85740; CHK1_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:Phospho-CHEK1 (Ser296)
IR:Immunogen Range:IQ(p-S)NL
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:1111
Swiss Prot:O14757
Source:KLH conjugated Synthesised phosphopeptide derived from human CHEK1 isoform b around the phosphorylation site of Ser296:IQ(p-S)NL
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011].
Size:100ul
Concentration:1mg/ml
Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Dog
Pig
Cow
Horse
Rabbit
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (Human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-CHEK1 (Ser296)) Polyclonal Antibody, Unconjugated (bs-11226R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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