Anti-VE Cadherin antibody (bs-4310R)

Anti-VE Cadherin antibody 

DATASHEET

Host:Rabbit

Target Protein:VE Cadherin

IR:Immunogen Range:251-320/784

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:1003

Swiss Prot:P33151

Source:KLH conjugated synthetic peptide derived from human VE Cadherin:251-320/784 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene is a classical cadherin from the cadherin superfamily and is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq, Jul 2008].

Size:100ul

Concentration:1mg/ml

Applications:Flow-Cyt(1μg/Test)

Cross Reactive Species:Human
Mouse
Rat
Pig
Cow
Horse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Blank control (blue line): Mouse kidney (blue).
Primary Antibody (green line): Rabbit Anti-VE Cadherin antibody (bs-4310R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): F(ab’)2 fragment goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃.Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.