Anti-ARNTL2 antibody (bs-11446R)

Anti-ARNTL2 antibody

DATASHEET

Host:Rabbit

Target Protein:ARNTL2

IR:Immunogen Range:381-480/636

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:56938

Swiss Prot:Q8WYA1

Source:KLH conjugated synthetic peptide derived from human ARNTL2:381-480/636 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:BMAL2 is a 636 amino acid protein that localizes to the nucleus and contains one bHLH (basic helix-loop-helix) domain, one PAC (PAS-associated C-terminal) domain and two PAS (PER-ARNT-SIM) domains. Expressed at high levels in placenta and brain and at lower levels in liver, thymus, heart, lung and kidney, BMAL2 functions as a component of the circadian core oscillator, which includes a variety of proteins that work in tandem to activate the transcription of target genes. More specifically, BMAL2, when functioning as a component of the core oscillator, binds to the E-box element (3'-CACGTG-5') of target DNA, thus inducing transcription. Multiple isoforms of BMAL2 exist due to alternative splicing events.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(2ug/test)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lane 1: Testis (Mouse) Lysate at 40 ug
Lane 2: Testis (Rat) Lysate at 40 ug
Primary: Anti-ARNTL2 (bs-11446R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 71/63 kD
Observed band size: 65 kD

Blank control:A431.
Primary Antibody (green line): Rabbit Anti-ARNTL2 antibody (bs-11446R-FITC)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.

Blank control:A431.
Primary Antibody (green line): Rabbit Anti-ARNTL2 antibody (bs-11446R-Cy5)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.