Anti-Semaphorin 5B antibody (bs-11482R)

Anti-Semaphorin 5B antibody

DATASHEET

Host:Rabbit

Target Protein:Semaphorin 5B

IR:Immunogen Range:146-195/1151

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:54437

Swiss Prot:Q9P283

Source:KLH conjugated synthetic peptide derived from human SEMA5B/Semaphorin 5B:146-195/1151 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Semaphorins are a family of cell surface and secreted proteins that are conserved from insects to humans. Members of this family of proteins are approximately 750 amino acids in length (including signal sequences) and are defined by a conserved extracellular “semaphorin” domain of approximately 500 amino acids containing 14-16 cysteines, blocks of conserved sequences and no obvious repeats. Secreted and cell-bound semaphorins chemically attract and repel the growth of neural axons, guiding the development of intricate networks of neural tissue. SEMA5B (semaphorin-5B), also known as SEMAG, is a 1151 amino acid member of the semaphorin family. Localized to the membrane, SEMA5B is a single-pass type III protein that functions to convey positive axonal guidance cues and can induce calpain-mediated cleavage of calcineurin. Three isoforms exist due to alternative splicing events.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Cow
Horse
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Testis (Mouse) Lysate at 40 ug
Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti-Semaphorin 5B (bs-11482R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 126 kD
Observed band size: 126/150 kD