Anti-NPW/Neuropeptide W antibody (bs-11531R)

Anti-NPW/Neuropeptide W antibody

DATASHEET

Host:Rabbit

Target Protein:NPW/Neuropeptide W

IR:Immunogen Range:33-55/165

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:283869

Swiss Prot:Q8N729

Source:KLH conjugated synthetic peptide derived from human Neuropeptide W:33-55/165 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:NPW is a 165 amino acid secreted protein that is cleaved into two chains: neuropeptide W-23 (also designated NPW23 or L8) and neuropeptide W-30 (also known as NPW30 or L8C). Both NPW neuropeptides activate G-protein coupled receptors in the central nervous system to enhance cortisol secretion. Highly expressed in lymphoblastic leukemia, colorectal adenocarcinoma, fetal kidney, trachea and substantia nigra, NPW is also found at low levels in placenta, ovary, testis and uterus. NPW functions in organization of neuroendocrine signals and is also thought to enhance food and water intake as well as stress responses. The gene encoding NPW maps to human chromosome 16p13.3.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Cow
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Neuropeptide W) Polyclonal Antibody, Unconjugated (bs-11531R) at 1:400 overnight at 4°C, followed by a conjugated secondary (bs-0295G-Cy3) at [1:500] for 90 minutes and DAPI staining of the nuclei.