Anti-LEO1 antibody
| 英文名称 | LEO1 |
| 中文名称 | RNA聚合酶相关蛋白LEO1抗体 |
| 别 名 | Gm185; LOC123439; Paf1/RNA polymerase II complex component; Replicative senescence down regulated leo1 like protein; Replicative senescence downregulated leo1 like; RNA polymerase associated protein LEO1; Senescence downregulated leo1 like; LEO1_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:LEO1
IR:Immunogen Range:451-550/666
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:123169
Swiss Prot:Q8WVC0
Source:KLH conjugated synthetic peptide derived from human LEO1:451-550/666
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:LEO1, parafibromin (CDC73; MIM 607393), CTR9 (MIM 609366), and PAF1 (MIM 610506) form the PAF protein complex that associates with the RNA polymerase II subunit POLR2A (MIM 180660) and with a histone methyltransferase complex (Rozenblatt-Rosen et al., 2005 [PubMed 15632063]).[supplied by OMIM, Mar 2008].
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
m.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-LEO1 Polyclonal Antibody, Unconjugated(bs-10030R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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