Anti-phospho-PKC Epsilon (Ser729) antibody
| 英文名称 | phospho-PKC Epsilon (Ser729) |
| 中文名称 | 磷酸化蛋白激酶C/p-PKC ε抗体 |
| 别 名 | PKC epsilon (phospho S729); PKC Epsilon (phospho Ser729); p-PKC Epsilon (Ser729); PKC-A; PKC alpha; AAG6; Aging associated gene 6; PICK 1; PKC A; PKCA; PKC A; PRKCA; Protein Kinase C alpha; Protein kinase C alpha type; KPCE_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:phospho-PKC Epsilon (Ser729)
IR:Immunogen Range:FS(p-Y)FG
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:5581
Swiss Prot:Q02156
Source:KLH conjugated synthesised phosphopeptide derived from human PKCE around the phosphorylation site of Ser729:FS(p-Y)FG
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior. [provided by RefSeq, Jul 2008].
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p-PKC Epsilon(Ser729)) Polyclonal Antibody, Unconjugated (bs-8608R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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