Anti-RTP3 antibody (bs-11619R)

Anti-RTP3 antibody

DATASHEET

Host:Rabbit

Target Protein:RTP3

IR:Immunogen Range:21-120/232

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:83597

Swiss Prot:Q9BQQ7

Source:KLH conjugated synthetic peptide derived from human RTP3:21-120/232 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Members of the RTP (receptor transporter proteins) family have recently been discovered to influence bitter taste receptor expression in addition to inducing the expression of mammalian odorant receptors. RTP3 is a 232 amino acid single-pass type III membrane protein belonging to the TMEM7 family. Unlike other RTP proteins, RTP3 is not expressed in olfactory neurons but is expressed predominantly in liver. RTP3 is involved in the functional expression of bitter taste receptors and suppresses cell proliferation, and is also found in human circumvallate papillae and testis (regions where bitter taste receptors are expressed). The gene encoding RTP3 maps to human chromosome 3p21.31 within C3CER1 (chromosome 3 common eliminated region 1), which is frequently eliminated in chromosomal deletions of solid tumors.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
Flow-Cyt(0.2ug/test)

Cross Reactive Species:Human
Mouse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Blank control:Molt-4.
Primary Antibody (green line): Rabbit Anti-RTP3 antibody (bs-11619R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.