Anti-ADAM12 antibody (bs-4977R)

Anti-ADAM12 antibody

DATASHEET

Host:Rabbit

Target Protein:ADAM12

IR:Immunogen Range:251-350/909

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:8038

Swiss Prot:O43184

Source:KLH conjugated synthetic peptide derived from human ADAM12/MLTN:251-350/909 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:ADAM (a disintegrin and metalloprotease) proteins are a family of over 30 membrane-anchored, glycosylated, Zn2+ dependent proteases that are involved in cell-cell, cell-matrix interface related processes including fertilization, muscle fusion, secretion of TNF?(tumor necrosis factor ?, and modulation of the neurogenic function of Notch and Delta (1-3). ADAM proteins possess a signal-domain, a pro-domain, a metalloprotease domain, a disintegrin domain (Integrin ligand), a cysteine-rich region, an epidermal growth factor-like domain, a transmembrane domain and a cytoplasmic tail (1-3). ADAMs are expressed in brain, testis, epididymis, ovary, breast, placenta, liver, heart, lung, bone, and muscle, and catalyze proteolysis, adhesion, fusion, and intracellular signaling (3). ADAM 12 (Meltrin-a) is produced as 2 differentially spliced isoforms, a 718 amino acid secreted form (ADAM12S) and a 881 amino acid membrane-bound form (ADAM12L), and is involved in egg-sperm fusion (4-6).

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: Placenta (Mouse) Lysate at 40 ug
Primary: Anti-ADAM12 (bs-4977R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 77/100 kD
Observed band size: 77 kD