Anti-GPR116 antibody (bs-12025R)

Anti-GPR116 antibody

DATASHEET

Host:Rabbit

Target Protein:GPR116

IR:Immunogen Range:501-600/1346

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:221395

Swiss Prot:Q8IZF2

Source:KLH conjugated synthetic peptide derived from human G protein coupled receptor 116:501-600/1346 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:G protein-coupled receptors (GPRs), also known as seven transmembrane receptors, heptahelical receptors or 7TM receptors, comprise a superfamily of proteins that play a role in many different stimulus-response pathways. G protein coupled receptors translate extracellular signals into intracellular signals (G protein activation) and they respond to a variety of signaling molecules, such as hormones and neurotransmitters. GPR116 (G protein-coupled receptor 116) is a 1,346 amino acid multi-pass membrane protein that contains one SEA domain, one GPS domain and three Ig-like domains and belongs to the GPR family. Existing as a dilsulfide-liked homodimer at the cell surface, GPR116 exists as multiple alternatively spliced isoforms and is thought to play a role in regulating and maintaining proper acid-base balance throughout the cell.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)

Cross Reactive Species:Human
Mouse
Rat
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Blank control (blue line): Hela (Black).
Primary Antibody (green line): Rabbit Anti-GPR116 antibody (bs-12025R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% paraformaldehyde for 10 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature.The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.