Anti-ZFX antibody (bs-12306R)

Anti-ZFX antibody

DATASHEET

Host:Rabbit

Target Protein:ZFX

IR:Immunogen Range:401-500/805

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:7543

Swiss Prot:P17010

Source:KLH conjugated synthetic peptide derived from human ZFX:401-500/805 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Krüppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. Zfy1 (zinc finger protein 1, Y linked), also known Zfy-1 is a 782 amino acid nuclear protein belonging to the Krüppel C2H2-type zinc-finger protein family and the ZFX/ZFY subfamily. Containing thirteen C2H2-type zinc fingers, Zfy1 is expressed in the genital ridge and adult testis and may be a probable transcription activator. The gene encoding Zfy1 maps to mouse chromosome Y.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Cow
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ZFX Polyclonal Antibody, Unconjugated(bs-12306R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining