Anti-Protein BOC antibody (bs-12322R)

Anti-Protein BOC antibody

DATASHEET

Host:Rabbit

Target Protein:Protein BOC

IR:Immunogen Range:451-550/1114

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:91653

Swiss Prot:Q9BWV1

Source:KLH conjugated synthetic peptide derived from human Protein BOC:451-550/1114 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:BOC is a receptor-like, single pass membrane protein belonging to the cell surface molecule subfamily of the Immunoglobulin/fibronectin type-III repeat family within the immunoglobulin superfamily. It contains three fibronectin type-III domains and four Immunoglobulin-like C2-type domains in its extracellular region. The intracellular region of BOC is not required for proper function. BOC localizes to the cell membrane and is ubiquitously expressed with highest expression levels in skeletal muscle and small intestine. Its mRNA expression is down-regulated by Ras. BOC is involved in accelerating myoblast differentiation and is dependent on CDO for its activity. BOC and CDO are co-expressed in muscle precursors and are components of a receptor complex that mediates cell-cell interactions important in myogenesis. Overexpression of BOC results in enhanced differentiation of myoblast cells. In addition, BOC is a target and signaling component of the Shh pathway.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Horse
Zebrafish
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Tissue/cell: rat muscle tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Protein BOC Polyclonal Antibody, Unconjugated(bs-12322R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei