Anti-5T4 antibody (bs-12348R)

Anti-5T4 antibody 

DATASHEET

Host:Rabbit

Target Protein:5T4

IR:Immunogen Range:31-100/420

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:7162

Swiss Prot:Q13641

Source:KLH conjugated synthetic peptide derived from human TPBG/5T4:31-100/420 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:TPBG is a 420 amino acid single-pass type I membrane protein expressed by all types of trophoblasts as early as 9 weeks of development. TPBG contains an N-terminal putative signal sequence, a 310-residue extracellular region, a membrane anchorage domain and a 44-amino acid cytoplasmic tail with a potential phosphorylation site. The extracellular region has seven potential N-glycosylation sites and seven leucine-rich repeats, which are located in two regions separated by a hydrophilic stretch. Suggested to be involved in cell adhesion, TPBG may also be associated with tumor growth and progression.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Cow
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
HepG2 Cell (Human) Lysate at 30 ug
A549 Cell (Human) Lysate at 30 ug
Primary: Anti-5T4 (bs-12348R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 63 kD