Anti-PCK1 antibody
英文名称 | PCK1 |
中文名称 | 磷酸烯醇丙酮酸羧激酶抗体 |
别 名 | PCK1; PEPC; GTP; MGC22652; PEP carboJNCkinase; PEPCK 1; PEPCK1; PEPCKC; Phosphoenolpyruvate carboJNCkinase 1; Phosphoenolpyruvate carboJNClase; Phosphopyruvate carboJNClase. |
DATASHEET
Host:Rabbit
Target Protein:PCK1
IR:Immunogen Range:201-300/622
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:5105
Swiss Prot:P35558
Source:KLH conjugated synthetic peptide derived from human PCK1:201-300/622
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:PCK1 is a main control point for the regulation of gluconeogenesis. This cytosolic enzyme, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of the corresponding gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. A mitochondrial isozyme has also been characterized.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Rabbit
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti-PCK1 (bs-4972R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 69 kD
Observed band size: 66 kD
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