Anti-Casein kinase 1 gamma 2 antibody
| 英文名称 | Casein kinase 1 gamma 2 |
| 中文名称 | 酪蛋白激酶1γ2/casein kinase Iγ1抗体 |
| 别 名 | Casein kinase 1 gamma 2; Casein kinase I isoform gamma 1; Casein kinase I isoform gamma 3; Casein kinase I isoform gamma-2; CK1g2; CKI gamma 1; CKI gamma 2; CKI gamma 3; CSNK1G1; CSNK1G2; casein kinase Iγ1; CSNK1G3; KC1G2_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:Casein kinase 1 gamma 2
IR:Immunogen Range:201-300/415
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:1455
Swiss Prot:P78368
Source:KLH conjugated synthetic peptide derived from human CK1g2/Casein Kinase 1 gamma 2:201-300/415
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. They can phosphorylate a large number of proteins. Participates in Wnt signaling.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(2ug/Test)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Blank control:Mouse kidney.
Primary Antibody (green line): Rabbit Anti-Casein kinase 1 gamma 2 antibody (bs-12425R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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