Anti-Phospho-Aurora B (Tyr12) antibody
| 英文名称 | Phospho-Aurora B (Tyr12) |
| 中文名称 | 磷酸化有丝分裂激酶B抗体 |
| 别 名 | Aurora B (phospho Y12); AURKB_HUMAN; Aurora B (phospho T12); AIK2; AIM1; ARK2; AurB; AURKB; Aurora 1; Aurora and Ipl1 like midbody associated protein 1; Aurora kinase B; Aurora related kinase 2; Aurora-B; Aurora/IPL1 related kinase 2; IPL1; Serine/theronine kinase 12; Serine/threonine protein kinase 12; STK1; STK12; STK5. |
DATASHEET
Host:Rabbit
Target Protein:Phospho-Aurora B (Tyr12)
IR:Immunogen Range:WP(p-Y)GR
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:9212
Swiss Prot:Q96GD4
Source:KLH conjugated Synthesised phosphopeptide derived from human Aurora B around the phosphorylation site of Tyr12:WP(p-Y)GR
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Mahogany (MG), originally identified as a protein involved in pigmentation, acts in conjunction with melanocortin receptors to suppress diet-induced obesity. Mahogany contains a single transmembrane domain, and it is expressed in a broad range of tissues, including the hypothalamus and pigment cells. Mutations within the mahogany gene were shown to rescue agouti-lethal-yellow mutant mice from obesity. The extracellular domain of mouse mahogany is the ortholog of the human protein attractin. Attractin (also designated DPPT-L) is a human serum glycoprotein and is a member of the CUB family of cell adhesion and guidance proteins. Attractin is expressed on activated T cells and is released from the cells 48 to 72 hours after activation.
Size:100ul
Concentration:1mg/ml
Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Pig
Cow
Horse
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Aurora B (Tyr12)) Polyclonal Antibody, Unconjugated (bs-12552R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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