Anti-DNASE1L1 antibody
| 英文名称 | DNASE1L1 |
| 中文名称 | DNA酶1样蛋白1抗体 |
| 别 名 | DeoJNCribonuclease I-like 1; DeoJNCribonuclease I like 1; DeoJNCribonuclease-1-like 1; DNAS1L1; DNase I like 1; DNase I like muscle specific; DNase I lysosomal like; DNase I-like 1; DNase X; DNASE1L1; DNASEX; DNL1L; DNSL1_HUMAN; Muscle specific DNase I like; DNASEX; Muscle-specific DNase I-like; XIB. |
DATASHEET
Host:Rabbit
Target Protein:DNASE1L1
IR:Immunogen Range:201-302/302
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:1774
Swiss Prot:P49184
Source:KLH conjugated synthetic peptide derived from human DNASE1L1:201-302/302
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:DNASE1L1 is a 302 amino acid protein that localizes to the endoplasmic reticulum and belongs to the deoJNCribonuclease family. Expressed at high levels in cardiac and skeletal muscle and at lower levels in a variety of tissues throughout the body, DNASE1L1 exists as multiple alternatively spliced isoforms and is thought to function in a similar manner to DNase l, possibly mediating internucleosomal DNA degradation via catalytic cleavage events. The gene encoding DNASE1L1 maps to human chromosome X, which contains nearly 153 million base pairs and houses over 1,000 genes. In conjunction with chromosome Y, chromosome X is responsible for sex determination. There are a number of conditions related to an abnormal number and combination of sex chromosomes, some of which include Turner's syndrome, color blindness, hemophilia and Duchenne muscular dystrophy.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
Flow-Cyt(2ug/Test)
Cross Reactive Species:Human
Mouse
Rat
Dog
Horse
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Blank control:Mouse kidney.
Primary Antibody (green line): Rabbit Anti-DNASE1L1 antibody (bs-12995R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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