Anti-phospho-EPO Receptor (Tyr485) antibody (bs-13088R)

Anti-phospho-EPO Receptor (Tyr485) antibody

DATASHEET

Host:Rabbit

Target Protein:phospho-EPO Receptor (Tyr485)

IR:Immunogen Range:GP(p-Y)SN

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:2057

Swiss Prot:P19235

Source:KLH conjugated synthesised phosphopeptide derived from human EPO Receptor around the phosphorylation site of Tyr485:GP(p-Y)SN 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The erythropoietin receptor (EPOR) is a member of the cytokine receptor family. There are several isoforms including: EPOR-F (full length), EPOR-S (soluble form), and EPOR-T (truncated form). Upon erythropoietin (EPO) binding, the EPOR activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated EPOR appears to have a role in erythroid cell survival. Defects in the EPOR may produce erythroleukemia and familial erythrocytosis. A functional EPOR is found in the cardiovascular system, including endothelial cells and cardiomyocytes, and data suggest that the EPO/EPO receptor system plays an important role in cardiac function. In animal studies, treatment with EPO during ischemia/reperfusion in the heart has been shown to limit the infarct size and the extent of apoptosis.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)

Cross Reactive Species:Human
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Hela(Human) Cell Lysate at 30 ug
HL-60(Human) Cell Lysate at 30 ug
MOLT-4(Human) Cell Lysate at 30 ug
Primary: Anti- phospho-EPO Receptor (Tyr485) (bs-13088R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD