Anti-Phospho-HER2 (Tyr1221) antibody
| 英文名称 | Phospho-HER2 (Tyr1221) |
| 中文名称 | 磷酸化HER2受体抗体 |
| 别 名 | ErbB 2 (phospho Y1221); p-ErbB 2 (phospho Y1221); HER2(Phospho-Tyr1221); ErbB2; CerbB2; c erb B2; C erbB 2; C-erbB2; CD340; CD340 antigen; Cerb B2/neu protein; Erb B2; erbb2; HER 2; HER 2/neu; HER2; Her2/neu; Herstatin; MLN 19; MLN19; NEU; NEU Proto Oncogene; Neuro Glioblastoma Derived Oncogene Homolog; Neuroblastoma/glioblastoma derived oncogene homolog; NGL; p185 ErbB2; p185erbB2; Receptor Protein Tyrosine Kinase ErbB2 Precursor; Receptor tyrosine protein kinase erbB 2; TKR1; ERBB2_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:Phospho-HER2 (Tyr1221)
IR:Immunogen Range:NL(p-Y)YW
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:2064
Swiss Prot:P04626
Source:KLH conjugated Synthesised phosphopeptide derived from human HER2 around the phosphorylation site of Tyr1221:NL(p-Y)YW
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008].
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
ICC(1:100)
Cross Reactive Species:Human
Mouse
Dog
Pig
Horse
Rabbit
Guinea Pig
dog.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Tissue/cell:A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (Phospho-HER2 (Tyr1221)) polyclonal Antibody, Unconjugated (bs-13090R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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