Anti-Ferredoxin Reductase antibody
| 英文名称 | Ferredoxin Reductase |
| 中文名称 | 铁氧化还原蛋白还原酶抗体 |
| 别 名 | adrenodoxin reductase; ADRO_HUMAN; ADXR; AR; FDXR; Ferredoxin NADP(+) reductase; Ferredoxin reductase; Ferredoxin--NADP(+) reductase; mitochondrial; NADPH adrenodoxin oxidoreductase mitochondrial; NADPH:adrenodoxin oxidoreductase. |
DATASHEET
Host:Rabbit
Target Protein:Ferredoxin Reductase
IR:Immunogen Range:401-491/491
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:2232
Swiss Prot:P22570
Source:KLH conjugated synthetic peptide derived from human Ferredoxin Reductase:401-491/491
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Adrenodoxin Reductase (ADX Reductase) is a mitochondrial flavoprotein that receives electrons from NADPH and thereby initiates the electron-transport chain serving mitochondrial cytochromes P450. ADX Reductase participates in cholesterol side chain cleavage in all steroidogenic tissues, steroid 11-? hydroJNClation in the adrenal cortex, 25-OH-vitamin D3-24 hydroJNClation in the kidney and sterol C-27 hydroJNClation in the liver. Alternate splicing of ADX Reductase produces two isoforms. Human ADX Reductase maps to human chromosome 17q24-q25.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(2ug/Test)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Horse
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Blank control:Mouse kidney.
Primary Antibody (green line): Rabbit Anti-Ferredoxin Reductase antibody (bs-13154R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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