Anti-Supervillin antibody (bs-7123R)

Anti-Supervillin antibody 

DATASHEET

Host:Rabbit

Target Protein:Supervillin

IR:Immunogen Range:51-150/2214

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:6840

Swiss Prot:O95425

Source:KLH conjugated synthetic peptide derived from human Supervillin:51-150/2214 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Supervillin is an F-actin-binding protein originally with two reported isoforms of 205/250 kDa. The longer isoform, named archvillin, is muscle-specific. Supervillin is a tightly bound peripheral membrane protein that is concentrated at sites of epithelial cell-cell adhesion. It contributes to cell-cell adhesion, motility regulation, and information transfer between cell compartments. The COOH-terminus of supervillin is homologous to villin/gelsolin but is not responsible for the tight binding to the actin cytoskeleton in vivo. The NH2-terminus contains functional nuclear localization sequences and F-actin and myosin II binding domains. It has been suggested that supervillin may mediate actin and myosin II filament organization at cholesterol-rich membrane domains. Supervillin has been identified as a transcriptional activator of the androgen receptor. Supervillin is found in many cells of several species, but is most abundant in muscle, bone marrow, thyroid gland, and salivary gland.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Protein: mouse heart lysate;
Primary: rabbit Anti-Supervillin (bs-7123R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size:248 kD
Observed band size:248 kD