Anti-FCER1G antibody (bs-13167R)

Anti-FCER1G antibody

DATASHEET

Host:Rabbit

Target Protein:FCER1G

IR:Immunogen Range:10-86/86

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:2207

Swiss Prot:P30273

Source:KLH conjugated synthetic peptide derived from human FCER1G:10-86/86 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:IgE Fc Receptor I binds to the Fc region of immunoglobulins epsilon chain with high affinity, and is responsible for initiating the allergic response. Binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators such as histamines, responsible for the manifestations of allergy. IgE Fc Receptor I also induces the secretion of important lymphokines, effectors of the hypersensitivity response. It is a tetramer of a heavily glycosylated alpha chain, a beta chain, and two disulfide linked gamma chains. The gamma chains from Fc epsilon RI are also subunits of other Fc receptors. The gamma subunit is thought to be functionally significant in allowing the IgE Fc receptor to reach the cell surface. The cytoplasmic domains of the beta and gamma subunits each contain a conserved consesus sequence, ITAM, (immunoreceptor tyrosine activation motif). Phosphorylation of a pair of conserved tyrosine residues within this motif is required for signal transduction in mast cells and other hemopoietic cell types.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:Spleen (mouse)cell Lysate at 40 ug
Primary: Anti-FCER1G (bs-13167R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 10 kD
Observed band size: 15 kD