Anti-Phospho-FLT3 (Tyr599) antibody
| 英文名称 | Phospho-FLT3 (Tyr599) |
| 中文名称 | 磷酸化FMS样酪氨酸激酶3抗体 |
| 别 名 | Flt3 / CD135 (phospho Y599); p-CD135 (phospho Y599); p-Flt3(phospho Y599); Flt3 / CD135 (phospho Y599); p-Flt3 / CD135 (phospho Y599); p-Flt3 (phospho Y599); p-CD135 (phospho Y599); CD135 antigen; Fetal liver kinase 2; FL cytokine receptor; Flk 2; Flk2; Flt 3; Flt3; FMS like tyrosine kinase 3; Fms related tyrosine kinase 3; Growth factor receptor tyrosine kinase type III; Stem cell tyrosine kinase 1; Stk 1; Stk1; Tyrosine protein kinase receptor FLT3; FLT3_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:Phospho-FLT3 (Tyr599)
IR:Immunogen Range:YE(p-Y)DL
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:2322
Swiss Prot:P36888
Source:KLH conjugated Synthesised phosphopeptide derived from human FLT3 around the phosphorylation site of Tyr599:YE(p-Y)DL
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:CD135 is a tyrosine kinase receptor expressed on normal cells including CD34+ hematopoietic stem cells, myelomonocytic progenitors, primitive B cell progenitors, and thymocytes. CD135 is also expressed on malignant hematopoietic cells including AML, ALL and CML BC. CD135, also known as FMS-like tyrosine kinase 3, FLT3, STK1, and Flk2, is a growth factor receptor that binds the FLT3 ligand to promote the growth and differentiation of primitive hematopoietic cells. The intracytoplasmic domain of CD135 is modified by phosphorylation and has been shown to interact with Grb2, SOCS1, VAV1, and Shc. In humans, expression of Flt3 is restricted to subsets of CD34 positive as well as CD34 negative normal bone marrow cells. In these cells, the level of expression of Flt3 is rather low. Most of the CD34 bright Flt3+ cells co-express CD117 at high levels. They may represent early cycling, but not quiescent stem cells. Flt3+ cells in the CD34lo and CD34- populations do not co-express CD117 molecule and may represent B lymphoid precursors.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
Sheep
Guinea Pig
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
293T Cell(Human)Lysate at 30 ug
A549 Cell (Human) Lysate at 30 ug
Primary: Anti- p-FLT3(Tyr599) (bs-13184R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 109kD
Observed band size: 120kD
Blank control (Black line): Molt4 (Black).
Primary Antibody (green line):Rabbit Anti-Phospho-FLT3 (Tyr599) antibody (bs-13184R-AF647)
Dilution:3μg /10^6 cells;
Isotype Control Antibody (orange line):Rabbit IGg-AF647.
Dilution: 3μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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