Anti-GALM antibody (bs-13268R)

Anti-GALM antibody

DATASHEET

Host:Rabbit

Target Protein:GALM

IR:Immunogen Range:251-342/342

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:130589

Swiss Prot:Q96C23

Source:KLH conjugated synthetic peptide derived from human GALM/Galactose mutarotase:251-342/342 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Galactose Mutarotase is a member of the aldose epimerase family and is involved in hexose metabolism. Through its catalytic activity, Galactose Mutarotase converts beta-aldose to alpha-aldose on several sugars, including D-glucose, L-arabinose and D-JNClose. Found in the cytoplasm of most cells, Galactose Mutarotase plays a key role in galactose metabolism by catalyzing the conversion of beta-D-galactose to alpha-D-galactose. The enzyme contains two residues, Glu 304 and His 170, that are critical for catalysis, as well as His 96 and Asp 243, which are important for proper substrate recognition by the active site. No known diseases have been associated with mutations in the Galactose Mutarotase gene, although inhibition of Galactose Mutarotase activity could potentially be associated with a build-up of unmetabolized sugars during metabolism.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Pig
Cow
Horse
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GALM) Polyclonal Antibody, Unconjugated (bs-13268R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.